Tracking and trapping somatic mutations in Ig genes.
نویسنده
چکیده
منابع مشابه
Somatic hypermutation of the B cell receptor genes B29 (Igbeta, CD79b) and mb1 (Igalpha, CD79a).
Somatic hypermutation (SHM), coupled to selection by antigen, generates high-affinity antibodies during germinal center (GC) B cell maturation. SHM is known to affect Bcl6, four additional oncogenes in diffuse large B cell lymphoma, and the CD95Fas gene and is regarded as a major mechanism of B cell tumorigenesis. We find that mutations in the genes encoding the B cell receptor (BCR) accessory ...
متن کاملSomatic mutations within the untranslated regions of rearranged Ig genes in a case of classical Hodgkin's disease as a potential cause for the absence of Ig in the lymphoma cells.
Hodgkin-Reed-Sternberg (H-RS) cells are clonal B cells carrying Ig gene rearrangements. However, in situ hybridization methods failed to demonstrate Ig gene expression in H-RS cells of classical Hodgkin's disease (HD). Because somatic mutations rendering potentially functional Ig gene rearrangements nonfunctional were detected in some cases of the disease, it was speculated that H-RS cells in c...
متن کاملThe very 5′ end and the constant region of Ig genes are spared from somatic mutation because AID does not access these regions
Somatic hypermutation (SHM) is restricted to VDJ regions and their adjacent flanks in immunoglobulin (Ig) genes, whereas constant regions are spared. Mutations occur after about 100 nucleotides downstream of the promoter and extend to 1-2 kb. We have asked why the very 5' and most of the 3' region of Ig genes are unmutated. Does the activation-induced cytosine deaminase (AID) that initiates SHM...
متن کاملThe inference of antigen selection on Ig genes.
Analysis of somatic mutations in V regions of Ig genes is important for understanding various biological processes. It is customary to estimate Ag selection on Ig genes by assessment of replacement (R) as opposed to silent (S) mutations in the complementary-determining regions and S as opposed to R mutations in the framework regions. In the past such an evaluation was performed using a binomial...
متن کاملThe Role of DNA Repair in Somatic Hypermutation of Immunoglobulin Genes
I mmunoglobulin (Ig) genes are first modified through the V(D)J (V, variable; D, diversity; J, joining) recombina-tion process in pre–B cells. The Rag1/Rag2-dependent V(D)J recombination generates a large repertoire of B lym-phocytes, each expressing a unique antibody molecule. Rearranged Ig genes are further modified by the somatic hypermu-tation process in activated germinal center B lymphocy...
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ورودعنوان ژورنال:
- Journal of immunology
دوره 180 9 شماره
صفحات -
تاریخ انتشار 2008